diff --git a/tests/call_function_test.py b/tests/call_function_test.py
index 853af35d..04ecf169 100644
--- a/tests/call_function_test.py
+++ b/tests/call_function_test.py
@@ -39,7 +39,7 @@ class CallFunctionTest(testlib.RouterMixin, testlib.TestCase):
         self.local = self.router.local()
 
     def test_succeeds(self):
-        assert 3 == self.local.call(function_that_adds_numbers, 1, 2)
+        self.assertEqual(3, self.local.call(function_that_adds_numbers, 1, 2))
 
     def test_crashes(self):
         exc = self.assertRaises(mitogen.core.CallError,
@@ -47,13 +47,13 @@ class CallFunctionTest(testlib.RouterMixin, testlib.TestCase):
 
         s = str(exc)
         etype, _, s = s.partition(': ')
-        assert etype == 'exceptions.ValueError'
+        self.assertEqual(etype, 'exceptions.ValueError')
 
         msg, _, s = s.partition('\n')
-        assert msg == 'exception text'
+        self.assertEqual(msg, 'exception text')
 
         # Traceback
-        assert len(s) > 0
+        self.assertGreater(len(s), 0)
 
     def test_bad_return_value(self):
         exc = self.assertRaises(mitogen.core.StreamError,
@@ -61,7 +61,7 @@ class CallFunctionTest(testlib.RouterMixin, testlib.TestCase):
         self.assertEquals(exc[0], "cannot unpickle '__main__'/'CrazyType'")
 
     def test_returns_dead(self):
-        assert mitogen.core._DEAD == self.local.call(func_returns_dead)
+        self.assertEqual(mitogen.core._DEAD, self.local.call(func_returns_dead))
 
     def test_aborted_on_local_context_disconnect(self):
         stream = self.router._stream_by_id[self.local.context_id]
@@ -83,9 +83,9 @@ class CallFunctionTest(testlib.RouterMixin, testlib.TestCase):
 
     def test_accepts_returns_context(self):
         context = self.local.call(func_accepts_returns_context, self.local)
-        assert context is not self.local
-        assert context.context_id == self.local.context_id
-        assert context.name == self.local.name
+        self.assertIsNot(context, self.local)
+        self.assertEqual(context.context_id, self.local.context_id)
+        self.assertEqual(context.name, self.local.name)
 
 
 if __name__ == '__main__':
diff --git a/tests/channel_test.py b/tests/channel_test.py
index 2c6ae2be..d879341a 100644
--- a/tests/channel_test.py
+++ b/tests/channel_test.py
@@ -9,11 +9,11 @@ class ConstructorTest(testlib.RouterMixin, testlib.TestCase):
         # issue 32
         l1 = self.router.local()
         chan = mitogen.core.Channel(self.router, l1, 123)
-        assert chan.router == self.router
-        assert chan.context == l1
-        assert chan.dst_handle == 123
-        assert chan.handle is not None
-        assert chan.handle > 0
+        self.assertEqual(chan.router, self.router)
+        self.assertEqual(chan.context, l1)
+        self.assertEqual(chan.dst_handle, 123)
+        self.assertIsNotNone(chan.handle)
+        self.assertGreater(chan.handle, 0)
 
 
 if __name__ == '__main__':
diff --git a/tests/fakessh_test.py b/tests/fakessh_test.py
index 3b433b90..9e37945b 100644
--- a/tests/fakessh_test.py
+++ b/tests/fakessh_test.py
@@ -21,9 +21,9 @@ class RsyncTest(testlib.DockerMixin, unittest.TestCase):
             testlib.data_path('.'), 'target:/tmp/data'
         ])
 
-        assert return_code == 0
-        assert context.call(os.path.exists, '/tmp/data')
-        assert context.call(os.path.exists, '/tmp/data/simple_pkg/a.py')
+        self.assertEqual(return_code, 0)
+        self.assertTrue(context.call(os.path.exists, '/tmp/data'))
+        self.assertTrue(context.call(os.path.exists, '/tmp/data/simple_pkg/a.py'))
 
     def test_rsync_between_direct_children(self):
         # master -> SSH -> has-sudo-pubkey -> rsync(.ssh) -> master ->
@@ -52,9 +52,11 @@ class RsyncTest(testlib.DockerMixin, unittest.TestCase):
             'rsync', '--progress', '-vvva', '.ssh/', 'target:' + dest_path
         ])
 
-        assert return_code == 0
-        assert pubkey_acct.call(os.path.getsize, '.ssh/authorized_keys') == \
-               webapp_acct.call(os.path.getsize, dest_path + '/authorized_keys')
+        self.assertEqual(return_code, 0)
+        self.assertEqual(
+            pubkey_acct.call(os.path.getsize, '.ssh/authorized_keys'),
+            webapp_acct.call(os.path.getsize, dest_path + '/authorized_keys'),
+        )
 
 
 if __name__ == '__main__':
diff --git a/tests/id_allocation_test.py b/tests/id_allocation_test.py
index 0b32e3b3..2dae132c 100644
--- a/tests/id_allocation_test.py
+++ b/tests/id_allocation_test.py
@@ -9,7 +9,7 @@ class SlaveTest(testlib.RouterMixin, testlib.TestCase):
     def test_slave_allocates_id(self):
         context = self.router.local()
         id_ = context.call(id_allocation.allocate_an_id)
-        assert id_ == (self.router.id_allocator.next_id - 1)
+        self.assertEqual(id_, self.router.id_allocator.next_id - 1)
 
 
 if __name__ == '__main__':
diff --git a/tests/importer_test.py b/tests/importer_test.py
index 6d5753c1..489e24d6 100644
--- a/tests/importer_test.py
+++ b/tests/importer_test.py
@@ -39,8 +39,8 @@ class LoadModuleTest(ImporterMixin, testlib.TestCase):
     def test_module_added_to_sys_modules(self):
         self.context.send_await.return_value = self.response
         mod = self.importer.load_module(self.modname)
-        self.assertTrue(sys.modules[self.modname] is mod)
-        self.assertTrue(isinstance(mod, types.ModuleType))
+        self.assertIs(sys.modules[self.modname], mod)
+        self.assertIsInstance(mod, types.ModuleType)
 
     def test_module_file_set(self):
         self.context.send_await.return_value = self.response
@@ -50,12 +50,12 @@ class LoadModuleTest(ImporterMixin, testlib.TestCase):
     def test_module_loader_set(self):
         self.context.send_await.return_value = self.response
         mod = self.importer.load_module(self.modname)
-        self.assertTrue(mod.__loader__ is self.importer)
+        self.assertIs(mod.__loader__, self.importer)
 
     def test_module_package_unset(self):
         self.context.send_await.return_value = self.response
         mod = self.importer.load_module(self.modname)
-        self.assertTrue(mod.__package__ is None)
+        self.assertIsNone(mod.__package__)
 
 
 class LoadSubmoduleTest(ImporterMixin, testlib.TestCase):
@@ -96,7 +96,7 @@ class LoadModulePackageTest(ImporterMixin, testlib.TestCase):
     def test_module_loader_set(self):
         self.context.send_await.return_value = self.response
         mod = self.importer.load_module(self.modname)
-        self.assertTrue(mod.__loader__ is self.importer)
+        self.assertIs(mod.__loader__, self.importer)
 
     def test_module_path_present(self):
         self.context.send_await.return_value = self.response
@@ -111,7 +111,7 @@ class LoadModulePackageTest(ImporterMixin, testlib.TestCase):
     def test_module_data(self):
         self.context.send_await.return_value = self.response
         mod = self.importer.load_module(self.modname)
-        self.assertTrue(isinstance(mod.func, types.FunctionType))
+        self.assertIsInstance(mod.func, types.FunctionType)
         self.assertEquals(mod.func.__module__, self.modname)
 
 
diff --git a/tests/master_test.py b/tests/master_test.py
index 0f56c935..33b25565 100644
--- a/tests/master_test.py
+++ b/tests/master_test.py
@@ -36,7 +36,7 @@ class IterReadTest(unittest.TestCase):
         try:
             reader = self.func(proc.stdout.fileno())
             for i, chunk in enumerate(reader, 1):
-                assert i == int(chunk)
+                self.assertEqual(i, int(chunk))
                 if i > 3:
                     break
         finally:
@@ -52,7 +52,7 @@ class IterReadTest(unittest.TestCase):
                     got.append(chunk)
                 assert 0, 'TimeoutError not raised'
             except mitogen.core.TimeoutError:
-                assert len(got) == 0
+                self.assertEqual(len(got), 0)
         finally:
             proc.terminate()
 
@@ -68,7 +68,8 @@ class IterReadTest(unittest.TestCase):
             except mitogen.core.TimeoutError:
                 # Give a little wiggle room in case of imperfect scheduling.
                 # Ideal number should be 9.
-                assert 3 < len(got) < 5
+                self.assertLess(3, len(got))
+                self.assertLess(len(got), 5)
         finally:
             proc.terminate()
 
diff --git a/tests/module_finder_test.py b/tests/module_finder_test.py
index b4540dd8..e9477d4e 100644
--- a/tests/module_finder_test.py
+++ b/tests/module_finder_test.py
@@ -97,12 +97,12 @@ class GetModuleViaSysModulesTest(testlib.TestCase):
         # _socket comes from a .so
         import _socket
         tup = self.call('_socket')
-        self.assertEquals(None, tup)
+        self.assertIsNone(tup)
 
     def test_builtin_fails(self):
         # sys is built-in
         tup = self.call('sys')
-        self.assertEquals(None, tup)
+        self.assertIsNone(tup)
 
 
 class ResolveRelPathTest(testlib.TestCase):
diff --git a/tests/nested_test.py b/tests/nested_test.py
index 5b60a3e3..4ce58c06 100644
--- a/tests/nested_test.py
+++ b/tests/nested_test.py
@@ -13,7 +13,7 @@ class NestedTest(testlib.RouterMixin, testlib.TestCase):
             context = self.router.local(via=context, name='local%d' % x)
 
         pid = context.call(os.getpid)
-        self.assertTrue(isinstance(pid, int))
+        self.assertIsInstance(pid, int)
 
 
 if __name__ == '__main__':
diff --git a/tests/responder_test.py b/tests/responder_test.py
index bf9c64d9..5891b0ca 100644
--- a/tests/responder_test.py
+++ b/tests/responder_test.py
@@ -52,7 +52,7 @@ class BrokenModulesTest(unittest.TestCase):
         call = router.route.mock_calls[0]
         msg, = call[1]
         self.assertEquals(50, msg.handle)
-        self.assertTrue(msg.unpickle() is None)
+        self.assertIsNone(msg.unpickle())
 
     def test_ansible_six_messed_up_path(self):
         # The copy of six.py shipped with Ansible appears in a package whose
@@ -75,7 +75,7 @@ class BrokenModulesTest(unittest.TestCase):
         call = router.route.mock_calls[0]
         msg, = call[1]
         self.assertEquals(50, msg.handle)
-        self.assertTrue(isinstance(msg.unpickle(), tuple))
+        self.assertIsInstance(msg.unpickle(), tuple)
 
 
 if __name__ == '__main__':
diff --git a/tests/ssh_test.py b/tests/ssh_test.py
index 98bc0558..bdd6c825 100644
--- a/tests/ssh_test.py
+++ b/tests/ssh_test.py
@@ -53,7 +53,7 @@ class SshTest(testlib.DockerMixin, unittest.TestCase):
         except mitogen.ssh.PasswordError, e:
             pass
 
-        assert e[0] == self.stream_class.password_required_msg
+        self.assertEqual(e[0], self.stream_class.password_required_msg)
 
     def test_password_incorrect(self):
         try:
@@ -65,7 +65,7 @@ class SshTest(testlib.DockerMixin, unittest.TestCase):
         except mitogen.ssh.PasswordError, e:
             pass
 
-        assert e[0] == self.stream_class.password_incorrect_msg
+        self.assertEqual(e[0], self.stream_class.password_incorrect_msg)
 
     def test_password_specified(self):
         context = self.docker_ssh(
@@ -73,8 +73,10 @@ class SshTest(testlib.DockerMixin, unittest.TestCase):
             password='y',
         )
 
-        sentinel = 'i-am-mitogen-test-docker-image\n'
-        assert sentinel == context.call(plain_old_module.get_sentinel_value)
+        self.assertEqual(
+            'i-am-mitogen-test-docker-image\n',
+            context.call(plain_old_module.get_sentinel_value),
+        )
 
     def test_pubkey_required(self):
         try:
@@ -85,15 +87,17 @@ class SshTest(testlib.DockerMixin, unittest.TestCase):
         except mitogen.ssh.PasswordError, e:
             pass
 
-        assert e[0] == self.stream_class.password_required_msg
+        self.assertEqual(e[0], self.stream_class.password_required_msg)
 
     def test_pubkey_specified(self):
         context = self.docker_ssh(
             username='has-sudo-pubkey',
             identity_file=testlib.data_path('docker/has-sudo-pubkey.key'),
         )
-        sentinel = 'i-am-mitogen-test-docker-image\n'
-        assert sentinel == context.call(plain_old_module.get_sentinel_value)
+        self.assertEqual(
+            'i-am-mitogen-test-docker-image\n',
+            context.call(plain_old_module.get_sentinel_value),
+        )
 
 
 if __name__ == '__main__':
diff --git a/tests/utils_test.py b/tests/utils_test.py
index 20ca4541..c9c2241b 100644
--- a/tests/utils_test.py
+++ b/tests/utils_test.py
@@ -21,14 +21,14 @@ class RunWithRouterTest(unittest.TestCase):
 
     def test_run_with_broker(self):
         router = mitogen.utils.run_with_router(func0)
-        self.assertTrue(isinstance(router, mitogen.master.Router))
+        self.assertIsInstance(router, mitogen.master.Router)
         self.assertFalse(router.broker._thread.isAlive())
 
 
 class WithRouterTest(unittest.TestCase):
     def test_with_broker(self):
         router = func()
-        self.assertTrue(isinstance(router, mitogen.master.Router))
+        self.assertIsInstance(router, mitogen.master.Router)
         self.assertFalse(router.broker._thread.isAlive())