diff --git a/changelogs/fragments/ansible-test-venv-real-prefix.yaml b/changelogs/fragments/ansible-test-venv-real-prefix.yaml
new file mode 100644
index 00000000000..c74fb047e99
--- /dev/null
+++ b/changelogs/fragments/ansible-test-venv-real-prefix.yaml
@@ -0,0 +1,2 @@
+bugfixes:
+  - ansible-test - Fix Python real prefix detection when running in a ``venv`` virtual environment.
diff --git a/test/lib/ansible_test/_util/controller/tools/virtualenvcheck.py b/test/lib/ansible_test/_util/controller/tools/virtualenvcheck.py
index 90dfa39410e..4c542a23fde 100644
--- a/test/lib/ansible_test/_util/controller/tools/virtualenvcheck.py
+++ b/test/lib/ansible_test/_util/controller/tools/virtualenvcheck.py
@@ -5,10 +5,22 @@ __metaclass__ = type
 import json
 
 try:
+    # virtualenv <20
     from sys import real_prefix
 except ImportError:
     real_prefix = None
 
+try:
+    # venv and virtualenv >= 20
+    from sys import base_exec_prefix
+except ImportError:
+    base_exec_prefix = None
+
+try:
+    from sys import base_exec_prefix
+except ImportError:
+    base_exec_prefix = None
+
 print(json.dumps(dict(
-    real_prefix=real_prefix,
+    real_prefix=real_prefix or base_exec_prefix,
 )))