diff --git a/changelogs/fragments/ansible-test-constraints-cleanup.yaml b/changelogs/fragments/ansible-test-constraints-cleanup.yaml
new file mode 100644
index 00000000000..a928593c48a
--- /dev/null
+++ b/changelogs/fragments/ansible-test-constraints-cleanup.yaml
@@ -0,0 +1,2 @@
+minor_changes:
+  - ansible-test - Removed used ``MarkupSafe`` constraint for Python 3.5 and earlier.
diff --git a/test/lib/ansible_test/_data/requirements/constraints.txt b/test/lib/ansible_test/_data/requirements/constraints.txt
index e4ddd55a909..704d6da46bb 100644
--- a/test/lib/ansible_test/_data/requirements/constraints.txt
+++ b/test/lib/ansible_test/_data/requirements/constraints.txt
@@ -13,4 +13,3 @@ mock >= 2.0.0 # needed for features backported from Python 3.6 unittest.mock (as
 pytest-mock >= 1.4.0 # needed for mock_use_standalone_module pytest option
 setuptools < 45 ; python_version == '2.7' # setuptools 45 and later require python 3.5 or later
 pyspnego >= 0.1.6 ; python_version >= '3.10' # bug in older releases breaks on Python 3.10
-MarkupSafe < 2.0.0 ; python_version < '3.6' # MarkupSafe >= 2.0.0. requires Python >= 3.6