diff --git a/changelogs/fragments/51059-dnf-support-substitutions b/changelogs/fragments/51059-dnf-support-substitutions
new file mode 100644
index 00000000000..bc862913fb5
--- /dev/null
+++ b/changelogs/fragments/51059-dnf-support-substitutions
@@ -0,0 +1,3 @@
+minor_changes:
+  - dnf module now supports loading substitution overrides from the installroot
+
diff --git a/lib/ansible/modules/packaging/os/dnf.py b/lib/ansible/modules/packaging/os/dnf.py
index a4bfacbb810..3d91bc7ab15 100644
--- a/lib/ansible/modules/packaging/os/dnf.py
+++ b/lib/ansible/modules/packaging/os/dnf.py
@@ -530,6 +530,9 @@ class DnfModule(YumDnf):
         # Set installroot
         conf.installroot = installroot
 
+        # Load substitutions from the filesystem
+        conf.substitutions.update_from_etc(installroot)
+
         # Handle different DNF versions immutable mutable datatypes and
         # dnf v1/v2/v3
         #