diff --git a/changelogs/fragments/win_reg_stat-hku.yaml b/changelogs/fragments/win_reg_stat-hku.yaml
new file mode 100644
index 00000000000..5008dd1ef0d
--- /dev/null
+++ b/changelogs/fragments/win_reg_stat-hku.yaml
@@ -0,0 +1,2 @@
+bugfixes:
+- win_reg_stat - fix issue when trying to check keys in ``HKU:\`` - https://github.com/ansible/ansible/issues/59337
diff --git a/lib/ansible/modules/windows/win_reg_stat.ps1 b/lib/ansible/modules/windows/win_reg_stat.ps1
index aa7e4ba3147..c31fc5a8693 100644
--- a/lib/ansible/modules/windows/win_reg_stat.ps1
+++ b/lib/ansible/modules/windows/win_reg_stat.ps1
@@ -80,7 +80,7 @@ $registry_hive = switch(Split-Path -Path $path -Qualifier) {
     "HKCC:" { [Microsoft.Win32.Registry]::CurrentConfig }
     "HKCU:" { [Microsoft.Win32.Registry]::CurrentUser }
     "HKLM:" { [Microsoft.Win32.Registry]::LocalMachine }
-    "HKU" { [Microsoft.Win32.Registry]::Users }
+    "HKU:" { [Microsoft.Win32.Registry]::Users }
 }
 
 $key = $null
diff --git a/test/integration/targets/win_reg_stat/tasks/tests.yml b/test/integration/targets/win_reg_stat/tasks/tests.yml
index 285b501a4ad..5424ba4d45b 100644
--- a/test/integration/targets/win_reg_stat/tasks/tests.yml
+++ b/test/integration/targets/win_reg_stat/tasks/tests.yml
@@ -362,3 +362,16 @@
   assert:
     that:
     - not actual.exists
+
+# Tests https://github.com/ansible/ansible/issues/59337
+- name: test out all registry hives
+  win_reg_stat:
+    path: '{{ item }}'
+  register: reg_hive_stat
+  failed_when: not reg_hive_stat.exists
+  with_items:
+  - HKCR:\*
+  - HKCC:\Software
+  - HKCU:\Software
+  - HKLM:\Software
+  - HKU:\.DEFAULT