diff --git a/test/integration/host_vars/testhost b/test/integration/host_vars/testhost
index 62ac1d62918..4d04a96cbcf 100644
--- a/test/integration/host_vars/testhost
+++ b/test/integration/host_vars/testhost
@@ -6,8 +6,5 @@ role_var_beats_inventory: 'should_not_see_this'
 test_hash:
   host_vars_testhost: "this is in host_vars/testhost"
 
-# Support execution from within a virtualenv
-ansible_python_interpreter: '/usr/bin/env python'
-
 # Var precedence testing
 defaults_file_var_role3: "overridden from inventory"